This gene encodes an essential beta subunit for CLC chloride channels. These heteromeric channels localize to basolateral membranes of renal tubules and of potassium-secreting epithelia of the inner ear. Mutations in this gene have been associated with Bartter syndrome with sensorineural deafness.
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Recombinant fusion protein containing a sequence corresponding to amino acids 54-320 of human BSND (NP_476517.1).
Western blot analysis of extracts of various cell lines, using BSND antibody (144-07747) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat kidney using BSND antibody (144-07747) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using BSND antibody (144-07747) at dilution of 1:100 (40x lens).
12 months from the date of shipment when stored properly.