BTAF1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Accession Number | O14981 |
| Gene Id | 9044 |
| Gene Symbols | BTAF1 |
| Protein Name / Synonyms | BTAF1, MOT1, TAF(II)170, TAF172, TAFII170, TATA-binding protein-associated factor 172 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP, ChIP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:20 - 1:50
ChIP 1:20 - 1:100
IHC 1:50 - 1:200
IP 1:20 - 1:50
ChIP 1:20 - 1:100
Format
Calculated MW
76kDa/206kDa
Observed MW
250kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1600-1849 of human BTAF1 (NP_003963.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using BTAF1 antibody (144-05811) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug BTAF1 antibody (144-05811). Western blot was performed from the immunoprecipitate using BTAF1 antibody (144-05811) at a dilition of 1:500.
Chromatin immunoprecipitation analysis extracts of HeLa cells, using BTAF1 antibody (144-05811) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Expiration:
12 months from the date of shipment when stored properly.