Synonyms
CPAMD4 antibody, FLJ17816 antibody, FLJ17822 antibody, MGC142298 antibody, C5 antibody, complement C5 antibody, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 antibody, anaphylatoxin C5a analog antibody, complement component 5 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
188 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1223 and 1451 of C5 (Uniprot ID# P01031)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded hepatoma, using C5 antibody(107-10978) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: H1299
5% SDS PAGE
107-10978 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.