CA2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P00918 |
| Gene Id | 760 |
| Gene Symbols | CA2 |
| Protein Name / Synonyms | CA2, CA-II, CAC, CAII, Car2, HEL-76, HEL-S-282, carbonic anhydrase 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IF 1:10 - 1:100
IF 1:10 - 1:100
Format
Calculated MW
29kDa
Observed MW
27kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CA2 (NP_000058.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CA2 antibody (144-01440) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using CA2 antibody (144-01440). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.