CAD Polyclonal Antibody
As low as $166.00
In stock
Only %1 left

Product Description


Size 50 ul, 100 ul, 200 ul
Species Human
Host Species Rabbit
Clonality Polyclonal
Isotype Igg
Buffers 50% glycerol, PBS with 0.02% sodium azide, pH7.3.
Protein Name / Synonyms CAD, CDG1Z, EIEE50, GATD4, CAD protein
Accession Number P27708
Gene Symbols CAD
Gene Id 790
Purity Affinity purification


The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms.


WB 1:500 - 1:2000
IHC 1:50 - 1:100


Calculated MW
Observed MW


Recombinant fusion protein containing a sequence corresponding to amino acids 1650-1900 of human CAD (NP_004332.2).






Western blot analysis of extracts of various cell lines, using CAD antibody (144-08344) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - CAD antibody (144-08344)

Immunohistochemistry of paraffin-embedded human liver using CAD antibody (144-08344) at dilution of 1:100 (40x lens).
Immunohistochemistry - CAD antibody (144-08344)

Immunohistochemistry of paraffin-embedded human stomach using CAD antibody (144-08344) at dilution of 1:100 (40x lens).
Immunohistochemistry - CAD antibody (144-08344)

12 months from the date of shipment when stored properly.
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