WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Recombinant fusion protein containing a sequence corresponding to amino acids 462-691 of human CALCOCO1 (NP_065949.1).
Western blot analysis of extracts of various cell lines, using CALCOCO1 antibody (144-07987) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human kidney using CALCOCO1 antibody (144-07987) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using CALCOCO1 antibody (144-07987). Blue: DAPI for nuclear staining.
12 months from the date of shipment when stored properly.