CAPG Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P40121 |
| Gene Id | 822 |
| Gene Symbols | CAPG |
| Protein Name / Synonyms | CAPG, AFCP, HEL-S-66, MCP, macrophage-capping protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
36kDa/38kDa
Observed MW
38kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human CAPG (NP_001738.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CAPG Antibody (144-07324) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.![Western blot - [KO Validated] CAPG Polyclonal Antibody (144-07324)](https://cdn.sanity.io/images/g51potcz/production/eb64d890e5d77e30182ad57790f6eceded1c9001-231x350.jpg)
Western blot analysis of extracts of various cell lines, using CAPG Antibody (144-07324) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] CAPG Polyclonal Antibody (144-07324)](https://cdn.sanity.io/images/g51potcz/production/9b895ca5f643905d4a17cd83aead419896f5793a-176x350.jpg)
Western blot analysis of extracts from normal (control) and CAPG knockout (KO) HeLa cells, using CAPG antibody (144-07324) at 1:1000 dilution.![Western blot - [KO Validated] CAPG Polyclonal Antibody (144-07324)](https://cdn.sanity.io/images/g51potcz/production/8d99cc2812a3b6c0ffefbefcbb8884e954ea289c-350x350.jpg)
Immunohistochemistry of paraffin-embedded human lung cancer using CAPG Antibody (144-07324) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] CAPG Polyclonal Antibody (144-07324)](https://cdn.sanity.io/images/g51potcz/production/50e59753735b1b954f67174b2e3f0551083b918a-350x350.jpg)
Immunohistochemistry of paraffin-embedded human liver cancer using CAPG Antibody (144-07324) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] CAPG Polyclonal Antibody (144-07324)](https://www.raybiotech.com/files/images/Antibodies/144/144-07324/5.jpg)
Expiration:
12 months from the date of shipment when stored properly.