CAPN2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P17655 |
| Gene Id | 824 |
| Gene Symbols | CAPN2 |
| Protein Name / Synonyms | CAPN2, CANP2, CANPL2, CANPml, mCANP, calpain 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
71kDa/79kDa
Observed MW
80kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 350-680 of human CAPN2 (NP_001739.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CAPN2 antibody (144-01861) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.![Western blot - [KO Validated] CAPN2 Polyclonal Antibody (144-01861)](https://cdn.sanity.io/images/g51potcz/production/9fcd72b8b458a30127034807e25bb84acfd3a591-476x350.jpg)
Western blot analysis of extracts from normal (control) and CAPN2 knockout (KO) 293T cells, using CAPN2 antibody (144-01861) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] CAPN2 Polyclonal Antibody (144-01861)](https://cdn.sanity.io/images/g51potcz/production/262e4a225cdbd1b870272119cb4a3f1abb5341a9-368x550.jpg)
Immunofluorescence analysis of U2OS cells using CAPN2 antibody (144-01861) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] CAPN2 Polyclonal Antibody (144-01861)](https://www.raybiotech.com/files/images/Antibodies/144/144-01861/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.