Western blot analysis of extracts of various cell lines, using CAPRIN1 antibody (144-07910) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse brain using CAPRIN1 antibody (144-07910) (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
