Rabbit Anti-CASK (C-term) Antibody, 400 ul
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | O14936 |
| Gene Id | 8573 |
| Gene Symbols | CASK |
| Protein Name / Synonyms | CASK (C-term) |
| Clonality | Polyclonal |
| Preparation | This CASK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-596 amino acids from the C-terminal region of human CASK. |
| Concentration (lot specific) | 1.570 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK).
Specificity
Predicted Reactivity
Rat
Other Names
CASK; LIN2; Peripheral plasma membrane protein CASK; Calcium/calmodulin-dependent serine protein kinase; Protein lin-2 homolog
NCBI Accession #
NP_001119526.1;NP_001119527.1;NP_003679.2
Other Accession #
Q62915; O70589
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
105123
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of CASK (arrow) using Cask Antibody (C-term) (Cat# 102-17185). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CASK gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.