CASP3 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P42574 |
| Gene Id | 836 |
| Gene Symbols | CASP3 |
| Protein Name / Synonyms | CASP3, CPP32, CPP32B, SCA-1, caspase-3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Apoptosis, Cancer, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
31kDa
Observed MW
17kDa, 35kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human CASP3 (NP_004337.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of Jurkat cells, using CASP3 antibody (144-02156) at 1:1000 dilution. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Western blot analysis of extracts from normal (control) and CASP3 knockout (KO) 293T cells, using CASP3 antibody (144-02156) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.
Immunohistochemistry of paraffin-embedded rat brain using CASP3 antibody (144-02156) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using CASP3 antibody (144-02156) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using CASP3 antibody (144-02156) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using CASP3 antibody (144-02156) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using CASP3 antibody (144-02156) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using CASP3 antibody (144-02156) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using CASP3 antibody (144-02156) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using CASP3 antibody (144-02156) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.