Caspase3 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P42574 |
| Gene Id | 836 |
| Gene Symbols | CASP3 |
| Protein Name / Synonyms | CASP3, CPP32, CPP32B, SCA-1, caspase-3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Apoptosis, Cancer, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
31kDa
Observed MW
Refer to figures
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Caspase3 (NP_004337.2).
Modification
Unmodified
Storage
-20°C
Images
Immunohistochemistry of paraffin-embedded human lung cancer using CASP3 antibody (144-60067) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using CASP3 antibody (144-60067) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using CASP3 antibody (144-60067) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using CASP3 antibody (144-60067) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CASP3 antibody (144-60067) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using CASP3 antibody (144-60067) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using CASP3 antibody (144-60067) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CASP3 antibody (144-60067) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CASP3 antibody (144-60067) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CASP3 antibody (144-60067) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using CASP3 antibody (144-60067) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using CASP3 antibody (144-60067) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.