[KO Validated] CAST Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P20810 |
| Gene Id | 831 |
| Gene Symbols | CAST |
| Protein Name / Synonyms | CAST, BS-17, PLACK, calpastatin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
63kDa/71- 84kDa
Observed MW
130kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 550-650 of human CAST (NP_001177371.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CAST antibody (144-07634) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.![Western blot - [KO Validated] CAST Polyclonal Antibody (144-07634)](https://cdn.sanity.io/images/g51potcz/production/50685df479d23de53c6f3e174a8dfe55aa5ba7bc-373x350.jpg)
Western blot analysis of extracts from normal (control) and CAST knockout (KO) HeLa cells, using CAST antibody (144-07634) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] CAST Polyclonal Antibody (144-07634)](https://www.raybiotech.com/files/images/Antibodies/144/144-07634/2.jpg)
Expiration:
12 months from the date of shipment when stored properly.