CCM2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9BSQ5 |
| Gene Id | 83605 |
| Gene Symbols | CCM2 |
| Protein Name / Synonyms | CCM2, C7orf22, OSM, PP10187, CCM2 scaffolding protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a scaffold protein that functions in the stress-activated p38 Mitogen-activated protein kinase (MAPK) signaling cascade. The protein interacts with SMAD specific E3 ubiquitin protein ligase 1 (also known as SMURF1) via a phosphotyrosine binding domain to promote RhoA degradation. The protein is required for normal cytoskeletal structure, cell-cell interactions, and lumen formation in endothelial cells. Mutations in this gene result in cerebral cavernous malformations. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
39kDa/42kDa/48kDa/51kDa
Observed MW
55kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CCM2 (NP_113631.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of MCF7 cells, using CCM2 antibody (144-06544) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver using CCM2 antibody (144-06544) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using CCM2 antibody (144-06544) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using CCM2 antibody (144-06544) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.