CD163 Polyclonal Antibody
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q86VB7 |
| Gene Id | 9332 |
| Gene Symbols | CD163 |
| Protein Name / Synonyms | CD163, M130, MM130, SCARI1, CD163 molecule |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Dilutions
WB 1:500 - 1:1000
Format
Calculated MW
121kDa/124kDa/125kDa
Observed MW
180kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 750-1050 of human CD163 (NP_004235.4).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CD163 Antibody (144-08383) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Expiration:
12 months from the date of shipment when stored properly.