CD19 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P15391 |
| Gene Id | 930 |
| Gene Symbols | CD19 |
| Protein Name / Synonyms | CD19, B4, CVID3, CD19 molecule |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
61kDa
Observed MW
95kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 351-556 of human CD19 (NP_001761.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of Raji cells, using CD19 Antibody (144-02577) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat spleen using CD19 antibody (144-02577) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human vermiform appendix using CD19 antibody (144-02577) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using CD19 antibody (144-02577) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.