CD74 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Accession Number | P04233 |
| Gene Id | 972 |
| Gene Symbols | CD74 |
| Protein Name / Synonyms | CD74, DHLAG, HLADG, II, Ia-GAMMA, CD74 molecule |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cancer, Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
18kDa/26kDa/33kDa
Observed MW
29kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 73-232 of human CD74 (NP_004346.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CD74 antibody (144-05667) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human B cell lymphoma using CD74 Antibody (144-05667) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human amygdalitis using CD74 Antibody (144-05667) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human B cell lymphoma using CD74 Antibody (144-05667) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.