CDH2 Polyclonal Antibody
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P19022 |
| Gene Id | 1000 |
| Gene Symbols | CDH2 |
| Protein Name / Synonyms | CDH2, CD325, CDHN, CDw325, NCAD, cadherin-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.
Dilutions
WB 1:500 - 1:1000
IP 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
97kDa/99kDa
Observed MW
135kDa
Immunogen
A synthetic peptide of human CDH2
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CDH2 antibody (144-03045) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot analysis of extracts from normal (control) and CDH2 knockout (KO) HeLa cells, using CDH2 antibody (144-03045) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CDH2 antibody (144-03045). Western blot was performed from the immunoprecipitate using CDH2 antibody (144-03045) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.