CEBPG Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P53567 |
| Gene Id | 1054 |
| Gene Symbols | CEBPG |
| Protein Name / Synonyms | CEBPG, GPE1BP, IG/EBP-1, CCAAT/enhancer-binding protein gamma |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein: a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. The C/EBP family consist of several related proteins, C/EBP alpha, C/EBP beta, C/EBP gamma, and C/EBP delta, that form homodimers and that form heterodimers with each other. CCAAT/enhancer binding protein gamma may cooperate with Fos to bind PRE-I enhancer elements. Two transcript variants encoding the same protein have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
16kDa
Observed MW
16kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human CEBPG (NP_001797.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CEBPG antibody (144-07279) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using CEBPG antibody (144-07279) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using CEBPG antibody (144-07279) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using CEBPG antibody (144-07279) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using CEBPG antibody (144-07279) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using CEBPG antibody (144-07279).
Expiration:
12 months from the date of shipment when stored properly.