CHAF1B Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13112 |
| Gene Id | 8208 |
| Gene Symbols | CHAF1B |
| Protein Name / Synonyms | CHAF1B, CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7, chromatin assembly factor 1 subunit B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100
IHC 1:50 - 1:200
IF 1:20 - 1:100
Format
Calculated MW
61kDa
Observed MW
70kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 270-559 of human CHAF1B (NP_005432.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CHAF1B antibody (144-06259) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded mouse kidney using CHAF1B antibody (144-06259) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using CHAF1B antibody (144-06259) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using CHAF1B antibody (144-06259) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat fallopian tube using CHAF1B antibody (144-06259) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using CHAF1B antibody (144-06259) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using CHAF1B antibody (144-06259) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using CHAF1B antibody (144-06259). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.