This gene encodes GTPase-activating protein for ras-related p21-rac and a phorbol ester receptor. It is predominantly expressed in neurons, and plays an important role in neuronal signal-transduction mechanisms. Mutations in this gene are associated with Duane's retraction syndrome 2 (DURS2). Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CHN1 (NP_001813.1).
Western blot analysis of extracts of various cell lines, using CHN1 antibody (144-07546) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using CHN1 antibody (144-07546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using CHN1 antibody (144-07546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CHN1 antibody (144-07546) at dilution of 1:100 (40x lens).
12 months from the date of shipment when stored properly.