Rabbit Anti-CROT (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9UKG9 |
| Gene Id | 54677 |
| Gene Symbols | CROT |
| Protein Name / Synonyms | CROT (N-term) |
| Clonality | Polyclonal |
| Preparation | This CROT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-79 amino acids from the N-terminal region of human CROT. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | FC, IHC-P, Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
CROT encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.
Specificity
Other Names
CROT; COT; Peroxisomal carnitine O-octanoyltransferase
NCBI Accession #
NP_001137407.1;NP_001230674.1;NP_066974.2
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
70178
Recommended Dilutions
FC: 1:10-50; IHC: 1:50-100; WB: 1:1000
 | CROT Antibody (N-term)(Cat# 102-18252) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Formalin-fixed and paraffin-embedded human brain tissue reacted with CROT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Western blot analysis of CROT Antibody (N-term) (Cat# 102-18252) in K562 cell line and mouse liver tissue lysates (35ug/lane). CROT (arrow) was detected using the purified Pab. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.