CSNK1E Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P49674 |
| Gene Id | 1454 |
| Gene Symbols | CSNK1E |
| Protein Name / Synonyms | CSNK1E, CKIepsilon, HCKIE, casein kinase 1 epsilon |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
47kDa
Observed MW
47kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 247-416 of human CSNK1E (NP_689407.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CSNK1E antibody (144-01796) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] CSNK1E Polyclonal Antibody (144-01796)](https://cdn.sanity.io/images/g51potcz/production/2b8baa0d6246dbdd45f4985a7331ede915aef05d-357x350.jpg)
Western blot analysis of extracts from normal (control) and CSNK1E knockout (KO) HeLa cells, using CSNK1E antibody (144-01796) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.![Western blot - [KO Validated] CSNK1E Polyclonal Antibody (144-01796)](https://cdn.sanity.io/images/g51potcz/production/d9b6cbaa1fd1893ab65696f9e7c5afe076815a9d-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat pancreas using CSNK1E Antibody (144-01796) at dilution of 1:200 (40x lens).![Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (144-01796)](https://cdn.sanity.io/images/g51potcz/production/30356c2a9d82cd09eca67cf275168864bfc75121-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse brain using CSNK1E Antibody (144-01796) at dilution of 1:200 (40x lens).![Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (144-01796)](https://cdn.sanity.io/images/g51potcz/production/e4a116dd652ebb2b859f27ab1c4299312bc128ef-467x350.jpg)
Immunohistochemistry of paraffin-embedded rat pancreas using CSNK1E Antibody (144-01796) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (144-01796)](https://cdn.sanity.io/images/g51potcz/production/26c951639de90835a1d72ba7cf3fc48f3a901587-467x350.jpg)
Immunohistochemistry of paraffin-embedded mouse brain using CSNK1E Antibody (144-01796) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] CSNK1E Polyclonal Antibody (144-01796)](https://cdn.sanity.io/images/g51potcz/production/a9c92ca5a1baec09fb106c95a8a30e4c08e5ccc3-368x550.jpg)
Immunofluorescence analysis of HeLa cells using CSNK1E antibody (144-01796). Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] CSNK1E Polyclonal Antibody (144-01796)](https://www.raybiotech.com/files/images/Antibodies/144/144-01796/7.jpg)
Expiration:
12 months from the date of shipment when stored properly.
