CSPG4 Polyclonal Antibody
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q6UVK1 |
| Gene Id | 1464 |
| Gene Symbols | CSPG4 |
| Protein Name / Synonyms | CSPG4, HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2, chondroitin sulfate proteoglycan 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
250kDa
Observed MW
320kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1950-2210 of human CSPG4 (NP_001888.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CSPG4 antibody (144-03592) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] CSPG4 Polyclonal Antibody (144-03592)](https://cdn.sanity.io/images/g51potcz/production/e5d2ca401cf2f14bc44af8b3b5f31d37f8726378-298x350.jpg)
Western blot analysis of extracts from normal (control) and CSPG4 knockout (KO) HeLa cells, using CSPG4 antibody (144-03592) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.![Western blot - [KO Validated] CSPG4 Polyclonal Antibody (144-03592)](https://www.raybiotech.com/files/images/Antibodies/144/144-03592/2.jpg)
Expiration:
12 months from the date of shipment when stored properly.