CSPP1, CSPP, JBTS21, centrosome and spindle pole-associated protein 1
Accession Number
Q1MSJ5
Gene Symbols
CSPP1
Gene Id
79848
Recommended Applications
Western Blotting
Purity
Affinity purification
Background
This gene encodes a centrosome and spindle pole associated protein. The encoded protein plays a role in cell-cycle progression and spindle organization, regulates cytokinesis, interacts with Nephrocystin 8 and is required for cilia formation. Mutations in this gene result in primary cilia abnormalities and classical Joubert syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:1000
Format
Calculated MW
101kDa/141kDa/145kDa
Observed MW
146kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 962-1221 of human CSPP1 (NP_079066.5).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CSPP1 antibody (144-09983) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.
