Rabbit anti-Cyclin D1 (CCND1) Biotinylated Antibody, 50 ug
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Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | AAX36345 |
| Clonality | Polyclonal |
| Immunogen | The imunogen was a recombinant protein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 64, 000), Western Blotting (recommended work dilution= 1:1, 000-5, 000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein CCND1.The membrane blot was probed with antiCCND1 primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein CCND1 from E.coli cell (35kDa). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Cell Cycle |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Cyclin D1 (CCND1) is a critical mitogen-regulated cell-cycle control element whose transcriptional modulation plays a crucial role in cancer growth and progression. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat CCND1 has not been tested yet.
Related Products
Mouse Anti-Human Cyclin D1 (Cat: DS-MB-01076)
References
- Elena Beltran, et al. (2011) A cyclin-D1 interaction with BAX underlies its oncogenic role and potential as a therapeutic target in mantle cell lymphoma. Medical Sciences.26; 108(30): 1246112466.
- Tomoharu Kanie, et al. (2012) Genetic Reevaluation of the Role of F-Box Proteins in Cyclin D1 Degradation. Mol Cell Biol. 32(3): 590605.
Expiration:
12 months from the date of shipment when stored properly.
