The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL).
Dilutions
WB 1:500 - 1:2000 IHC 1:50 - 1:200
Format
Calculated MW
73kDa
Observed MW
74kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 406-645 of human DARS2 (NP_060592.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DARS2 antibody (144-07813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver injury using DARS2 antibody (144-07813) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using DARS2 antibody (144-07813) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using DARS2 antibody (144-07813) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
