Rabbit Anti-DCK (C-term) Antibody, 400 ul
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P27707 |
| Gene Id | 1633 |
| Gene Symbols | DCK |
| Protein Name / Synonyms | DCK (C-term) |
| Clonality | Polyclonal |
| Preparation | This DCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 amino acids from the C-terminal region of human DCK. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Deoxycytidine kinase is responsible for the phosphorylation of several deoxyribonucleosides and their analogs. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. It is the rate limiting enzyme in the activation of many important anticancer and retroviral drugs and its activity is often decreased in cells that are resistant to cytosine arabinoside.
Specificity
Other Names
DCK; Deoxycytidine kinase
NCBI Accession #
NP_000779.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
30519
Recommended Dilutions
IHC: 1:10-50; IF: 1:10-50; WB: 1:1000; IP: 1:500-1000
 | DCK Antibody (C-term) (Cat# 102-17119)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DCK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of DCK Antibody (C-term)(Cat# 102-17119) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
 | The anti-DCK Pab (Cat# 102-17119) is used in Western blot to detect DCK in mouse intestine tissue lysate. |
 | Deoxycytidine kinase(DCK) immunoprecipitated from H460cells with 7.5ug (microgram) of the dCK antibody (102-17119) using the Pierce classic mammalian IP kit (#45217) reagent as described as manufacturer instructions (lane 1, 3) and Current Protocols in Cell Biology, 1998, 7.2.1-7.2.21. Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with 1:700 dilution of DCK antibody (102-17119). Bands were detected using enhanced chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate Kit). No specific reagents were employed to remove IgG from immunoprecipitated sample. Data courtesy of Dr. Stacy Shord, University of Illinois, Chicago. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.