DDX1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q92499 |
| Gene Id | 1653 |
| Gene Symbols | DDX1 |
| Protein Name / Synonyms | DDX1, DBP-RB, UKVH5d, DEAD-box helicase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
IP 1:20 - 1:50
IHC 1:50 - 1:200
IF 1:10 - 1:100
IP 1:20 - 1:50
Format
Calculated MW
68kDa/69kDa/82kDa
Observed MW
100kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 441-740 of human DDX1 (NP_004930.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DDX1 antibody (144-06575) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using DDX1 Antibody (144-06575) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using DDX1 Antibody (144-06575) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DDX1 Antibody (144-06575) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using DDX1 Antibody (144-06575) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using DDX1 antibody (144-06575). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100ug extracts of 293T cells using 3ug DDX1 antibody (144-06575). Western blot was performed from the immunoprecipitate using DDX1 antibody (144-06575) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.