DDX41 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9UJV9 |
| Gene Id | 51428 |
| Gene Symbols | DDX41 |
| Protein Name / Synonyms | DDX41, ABS, MPLPF, DEAD-box helicase 41 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Concentration (lot specific) | 1.44 mg/ml |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD box protein family and interacts with several spliceosomal proteins. In addition, the encoded protein may recognize the bacterial second messengers cyclic di-GMP and cyclic di-AMP, resulting in the induction of genes involved in the innate immune response.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
Format
Calculated MW
69kDa
Observed MW
75kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DDX41 (NP_057306.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DDX41 antibody (144-06576) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using DDX41 antibody (144-06576) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using DDX41 antibody (144-06576) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using DDX41 antibody (144-06576) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using DDX41 antibody (144-06576). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug DDX41 antibody (144-06576). Western blot was performed from the immunoprecipitate using DDX41 antibody (144-06576) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.