DFNA5, ICERE-1, non-syndromic hearing impairment protein 5
Accession Number
O60443
Gene Symbols
DFNA5
Gene Id
1687
Recommended Applications
Western Blotting,IHC
Concentration (lot specific)
2.61 mg/ml
Purity
Affinity purification
Background
Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000 IHC 1:50 - 1:200
Format
Calculated MW
10kDa/36kDa/54kDa
Observed MW
50kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DFNA5 (NP_004394.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DFNA5 Antibody (144-07432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human liver injury using DFNA5 antibody (144-07432) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using DFNA5 antibody (144-07432) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using DFNA5 antibody (144-07432) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using DFNA5 antibody (144-07432) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
