EEF1B2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P24534 |
| Gene Id | 1933 |
| Gene Symbols | EEF1B2 |
| Protein Name / Synonyms | EEF1B2, EEF1B, EEF1B1, EF1B, elongation factor 1-beta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
24kDa
Observed MW
33kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human EEF1B2 (NP_001032752.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EEF1B2 antibody (144-06580) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] EEF1B2 Polyclonal Antibody (144-06580)](https://cdn.sanity.io/images/g51potcz/production/fe466f4578fc4a319387c7c390e0b76a03106a8a-335x350.jpg)
Western blot analysis of extracts from normal (control) and EEF1B2 knockout (KO) 293T cells, using EEF1B2 antibody (144-06580) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.![Western blot - [KO Validated] EEF1B2 Polyclonal Antibody (144-06580)](https://cdn.sanity.io/images/g51potcz/production/ea50a6c94c070a00e9f9972f5be3445c04655b15-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse brain using EEF1B2 Antibody (144-06580) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (144-06580)](https://cdn.sanity.io/images/g51potcz/production/80b089ec5314abdefd979fa2719a43ccfdaaaebe-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat spinal cord using EEF1B2 Antibody (144-06580) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (144-06580)](https://cdn.sanity.io/images/g51potcz/production/8fc14bb6571a8c14fa772c236fe9a6c0e787633a-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat brain using EEF1B2 Antibody (144-06580) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (144-06580)](https://cdn.sanity.io/images/g51potcz/production/26a0a6731e1908b519076573161261185ef191c3-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse brain using EEF1B2 Antibody (144-06580) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] EEF1B2 Polyclonal Antibody (144-06580)](https://cdn.sanity.io/images/g51potcz/production/c0c4fc1e9f9e6b44f7c34d150b3930a78e92726d-368x550.jpg)
Immunofluorescence analysis of A549 cells using EEF1B2 antibody (144-06580). Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] EEF1B2 Polyclonal Antibody (144-06580)](https://www.raybiotech.com/files/images/Antibodies/144/144-06580/7.jpg)
Expiration:
12 months from the date of shipment when stored properly.