EFNA1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P20827 |
| Gene Id | 1942 |
| Gene Symbols | EFNA1 |
| Protein Name / Synonyms | EFNA1, B61, ECKLG, EFL1, EPLG1, LERK-1, LERK1, TNFAIP4, ephrin-A1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
21kDa/23kDa
Observed MW
21-23kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 19-182 of human EFNA1 (NP_004419.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EFNA1 antibody (144-05341) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human prostate using EFNA1 Antibody (144-05341) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using EFNA1 Antibody (144-05341) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using EFNA1 Antibody (144-05341) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using EFNA1 Antibody (144-05341) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.