EIF3H Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O15372 |
| Gene Id | 8667 |
| Gene Symbols | EIF3H |
| Protein Name / Synonyms | EIF3H, EIF3S3, eIF3-gamma, eIF3-p40, eukaryotic translation initiation factor 3 subunit H |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Format
Calculated MW
39kDa
Observed MW
40kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EIF3H (NP_003747.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EIF3H antibody (144-013378) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat brain using EIF3H Antibody (144-013378) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using EIF3H Antibody (144-013378) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EIF3H Antibody (144-013378) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using EIF3H Antibody (144-013378) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using EIF3H antibody (144-013378). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug EIF3H antibody (144-013378). Western blot was performed from the immunoprecipitate using EIF3H antibody (144-013378) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.