EIF3J Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O75822 |
| Gene Id | 8669 |
| Gene Symbols | EIF3J |
| Protein Name / Synonyms | EIF3J, EIF3S1, eIF3-alpha, eIF3-p35, eukaryotic translation initiation factor 3 subunit J |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a core subunit of the eukaryotic initiation factor 3 complex, which participates in the initiation of translation by aiding in the recruitment of protein and mRNA components to the 40S ribosome. There are pseudogenes for this gene on chromosomes 1, 3, and 9. Alternative splicing results in multiple transcript variants encoding different isoforms.
Dilutions
WB 1:200 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
22kDa/23kDa/29kDa
Observed MW
37kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human EIF3J (NP_003749.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EIF3J antibody (144-08637) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using EIF3J antibody (144-08637) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using EIF3J antibody (144-08637) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EIF3J antibody (144-08637) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using EIF3J antibody (144-08637) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using EIF3J antibody (144-08637) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.