EIF3J Polyclonal Antibody
As low as $166.00
In stock
Only %1 left
Code:
144-08637
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Product Description

Specifications

Size 50 ul, 100 ul, 200 ul
Species Human, Mouse, Rat
Host Species Rabbit
Clonality Polyclonal
Isotype Igg
Buffers 50% glycerol, PBS with 0.02% sodium azide, pH7.3.
Protein Name / Synonyms EIF3J, EIF3S1, eIF3-alpha, eIF3-p35, eukaryotic translation initiation factor 3 subunit J
Accession Number O75822
Gene Symbols EIF3J
Gene Id 8669
Purity Affinity purification

Background

This gene encodes a core subunit of the eukaryotic initiation factor 3 complex, which participates in the initiation of translation by aiding in the recruitment of protein and mRNA components to the 40S ribosome. There are pseudogenes for this gene on chromosomes 1, 3, and 9. Alternative splicing results in multiple transcript variants encoding different isoforms.

Dilutions

WB 1:200 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW
22kDa/23kDa/29kDa
Observed MW
37kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human EIF3J (NP_003749.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using EIF3J antibody (144-08637) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - EIF3J Polyclonal Antibody (144-08637)

Immunohistochemistry of paraffin-embedded rat brain using EIF3J antibody (144-08637) at dilution of 1:100 (40x lens).
Immunohistochemistry - EIF3J Polyclonal Antibody (144-08637)

Immunohistochemistry of paraffin-embedded human lung using EIF3J antibody (144-08637) at dilution of 1:100 (40x lens).
Immunohistochemistry - EIF3J Polyclonal Antibody (144-08637)

Immunohistochemistry of paraffin-embedded human gastric cancer using EIF3J antibody (144-08637) at dilution of 1:100 (40x lens).
Immunohistochemistry - EIF3J Polyclonal Antibody (144-08637)

Immunohistochemistry of paraffin-embedded mouse spleen using EIF3J antibody (144-08637) at dilution of 1:100 (40x lens).
Immunohistochemistry - EIF3J Polyclonal Antibody (144-08637)

Immunofluorescence analysis of HeLa cells using EIF3J antibody (144-08637) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - EIF3J Polyclonal Antibody (144-08637)

Expiration:
12 months from the date of shipment when stored properly.
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