EIF4E Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P06730 |
| Gene Id | 1977 |
| Gene Symbols | EIF4E |
| Protein Name / Synonyms | EIF4E, AUTS19, CBP, EIF4E1, EIF4EL1, EIF4F, eIF-4E, eukaryotic translation initiation factor 4E |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
25kDa/27kDa/28kDa
Observed MW
25kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EIF4E (NP_001959.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EIF4E antibody (144-02162) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human lung cancer using EIF4E antibody (144-02162) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using EIF4E antibody (144-02162) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using EIF4E antibody (144-02162) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug EIF4E antibody (144-02162). Western blot was performed from the immunoprecipitate using EIF4E antibody (144-02162) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.