EIF4EBP1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13541 |
| Gene Id | 1978 |
| Gene Symbols | EIF4EBP1 |
| Protein Name / Synonyms | EIF4EBP1, 4E-BP1, 4EBP1, BP-1, PHAS-I, eukaryotic translation initiation factor 4E-binding protein 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | mTOR Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Dilutions
WB 1:500 - 1:1000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
12kDa
Observed MW
20kDa, 22kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human EIF4EBP1 (NP_004086.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EIF4EBP1 antibody (144-01248) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.![Western blot - [KO Validated] EIF4EBP1 Polyclonal Antibody (144-01248)](https://cdn.sanity.io/images/g51potcz/production/e6d850c347c85395d5ff19dcaa9bb0599b9ff798-411x350.jpg)
Western blot analysis of extracts from normal (control) and EIF4EBP1 knockout (KO) HeLa cells, using EIF4EBP1 antibody (144-01248) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.![Western blot - [KO Validated] EIF4EBP1 Polyclonal Antibody (144-01248)](https://www.raybiotech.com/files/images/Antibodies/144/144-01248/2.jpg)
Immunofluorescence analysis of NIH/3T3 cells using EIF4EBP1 antibody (144-01248) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] EIF4EBP1 Polyclonal Antibody (144-01248)](https://www.raybiotech.com/files/images/Antibodies/144/144-01248/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.