Rabbit Anti-EMX1 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q04741 |
| Gene Id | 2016 |
| Gene Symbols | EMX1 |
| Protein Name / Synonyms | EMX1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This EMX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-257 amino acids from the C-terminal region of human EMX1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
EMX1 is a transcription factor, which in cooperation with EMX2. It acts to generate the boundary between the roof and archipallium in the developing brain. It may function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
Specificity
Other Names
EMX1; Homeobox protein EMX1; Empty spiracles homolog 1; Empty spiracles-like protein 1
NCBI Accession #
NP_004088.2
Other Accession #
Q04742
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
28039
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IF: 1:10-50
 | EMX1 Antibody (C-term) (Cat# 102-18099) western blot analysis in U251 and Neuro-2a cell line mouse cerebellum tissue lysates (35ug/lane).This demonstrates the EMX1 antibody detected the EMX1 protein (arrow). |
 | EMX1 Antibody (C-term) (Cat# 102-18099) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Fluorescent confocal image of Hela cell stained with EMX1 Antibody (C-term)(Cat# 102-18099).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EMX1 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). EMX1 immunoreactivity is localized to Nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.