Rabbit Anti-EN2 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P19622 |
| Gene Id | 2020 |
| Gene Symbols | EN2 |
| Protein Name / Synonyms | EN2 (C-term) |
| Clonality | Polyclonal |
| Preparation | This EN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids from the C-terminal region of human EN2. |
| Concentration (lot specific) | 0.450 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
EN2 is thought to have a role in controlling development. In Drosophila, the protein plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. This protein has been implicated in the control of pattern formation during development of the central nervous system.
Specificity
Predicted Reactivity
Zebrafish, Chicken, Xenopus
Other Names
EN2; Homeobox protein engrailed-2
NCBI Accession #
NP_001418.2
Other Accession #
P09066; Q05917; P52730; P52729; P09015
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
34211
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | Western blot analysis of EN2 antibody (C-term) (Cat# 102-17362) in 293 cell line lysates and mouse stomach and lung tissue lysates (35ug/lane). EN2 (arrow) was detected using the purified Pab. |
 | Fluorescent confocal image of Hela cell stained with EN2 Antibody (C-term)(Cat# 102-17362). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). EN2 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.