EPB41 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P11171 |
| Gene Id | 2035 |
| Gene Symbols | EPB41 |
| Protein Name / Synonyms | EPB41, 4.1R, EL1, HE, protein 4.1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene, together with spectrin and actin, constitute the red cell membrane cytoskeletal network. This complex plays a critical role in erythrocyte shape and deformability. Mutations in this gene are associated with type 1 elliptocytosis (EL1). Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
63kDa/66kDa/71kDa/81kDa/86kDa/93kDa/97kDa
Observed MW
90kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 210-500 of human EPB41 (NP_001159478.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EPB41 antibody (144-02490) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human colon carcinoma using EPB41 antibody (144-02490) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EPB41 antibody (144-02490) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using EPB41 antibody (144-02490). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.