This gene encodes a protein which localizes to the lumen of the endoplasmic reticulum (ER). It is a member of the protein disulfide isomerase (PDI) protein family but lacks an active thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase. The canonical protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
5kDa/28kDa
Observed MW
29kDa
Immunogen
A synthetic peptide of human ERP29
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ERP29 antibody (144-07959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and ERP29 knockout (KO) 293T cells, using ERP29 antibody (144-07959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Expiration:
12 months from the date of shipment when stored properly.
![Western blot - [KO Validated] ERP29 Polyclonal Antibody (144-07959)](/files/images/Antibodies/144/144-07959/1.jpg "Western blot - [KO Validated] ERP29 Polyclonal Antibody (144-07959)")
![Western blot - [KO Validated] ERP29 Polyclonal Antibody (144-07959)](/files/images/Antibodies/144/144-07959/2.jpg "Western blot - [KO Validated] ERP29 Polyclonal Antibody (144-07959)")
