FDX1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P10109 |
| Gene Id | 2230 |
| Gene Symbols | FDX1 |
| Protein Name / Synonyms | FDX1, ADX, FDX, LOH11CR1D, ferredoxin 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to mitochondrial cytochrome P450, involved in steroid, vitamin D, and bile acid metabolism. Pseudogenes of this functional gene are found on chromosomes 20 and 21.
Dilutions
WB 1:200 - 1:500
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
19kDa
Observed MW
14kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human FDX1 (NP_004100.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using FDX1 antibody (144-09815) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using FDX1 antibody (144-09815) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using FDX1 antibody (144-09815) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using FDX1 antibody (144-09815) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using FDX1 antibody (144-09815) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using FDX1 antibody (144-09815) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.