The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.
Dilutions
WB 1:500 - 1:2000 IHC 1:50 - 1:100
Format
Calculated MW
47kDa/48kDa
Observed MW
50kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human FECH (NP_000131.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using FECH antibody (144-08418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human stomach using FECH antibody (144-08418) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using FECH antibody (144-08418) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using FECH antibody (144-08418) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using FECH antibody (144-08418) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
