FSCN1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q16658 |
| Gene Id | 6624 |
| Gene Symbols | FSCN1 |
| Protein Name / Synonyms | FSCN1, FAN1, HSN, SNL, p55, fascin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
54kDa
Observed MW
50kDa, 55kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 394-493 of human FSCN1 (NP_003079.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using FSCN1 antibody (144-01904) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.![Western blot - [KO Validated] FSCN1 Polyclonal Antibody (144-01904)](https://cdn.sanity.io/images/g51potcz/production/c0b71d4ece4e3103358c3d20aa7229920be482fc-512x350.jpg)
Western blot analysis of extracts from normal (control) and FSCN1 knockout (KO) HeLa cells, using FSCN1 antibody (144-01904) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.![Western blot - [KO Validated] FSCN1 Polyclonal Antibody (144-01904)](https://cdn.sanity.io/images/g51potcz/production/bd8c300f6c98294858ac1d97d204bb7b0fd08a4b-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat stomach using FSCN1 antibody (144-01904) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] FSCN1 Polyclonal Antibody (144-01904)](https://www.raybiotech.com/files/images/Antibodies/144/144-01904/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.