GATM Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P50440 |
| Gene Id | 2628 |
| Gene Symbols | GATM |
| Protein Name / Synonyms | GATM, AGAT, AT, CCDS3, glycine amidinotransferase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Mutations in this gene cause arginine:glycine amidinotransferase deficiency, an inborn error of creatine synthesis characterized by mental retardation, language impairment, and behavioral disorders.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
44kDa/48kDa
Observed MW
43kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 124-423 of human GATM (NP_001473.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GATM antibody (144-06598) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver using GATM Antibody (144-06598) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using GATM Antibody (144-06598) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using GATM antibody (144-06598) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using GATM antibody (144-06598) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using GATM antibody (144-06598) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using GATM antibody (144-06598) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using GATM antibody (144-06598) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.