Rabbit Anti-GCAT (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | O75600 |
| Gene Id | 23464 |
| Gene Symbols | GCAT |
| Protein Name / Synonyms | GCAT (Center) |
| Clonality | Polyclonal |
| Preparation | This GCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-181 amino acids from the Central region of human GCAT. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase.
Specificity
Predicted Reactivity
Bovine
Other Names
GCAT; KBL; 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial; Aminoacetone synthase; Glycine acetyltransferase
NCBI Accession #
NP_001165161.1;NP_055106.1
Other Accession #
O88986; Q0P5L8
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
45285
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of GCAT antibody (Center) (Cat# 102-17387) in mouse liver tissue lysates (35ug/lane). GCAT (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human brain reacted with GCAT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of HepG2 cells using GCAT Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Western blot analysis of GCAT (arrow) using rabbit polyclonal GCAT Antibody (Center) (Cat# 102-17387). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCAT gene. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.