[KO Validated] GIT1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9Y2X7 |
| Gene Id | 28964 |
| Gene Symbols | GIT1 |
| Protein Name / Synonyms | GIT1, GIT ArfGAP 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Dilutions
WB 1:200 - 1:2000
Format
Calculated MW
19kDa/84kDa/85kDa
Observed MW
100kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 460-640 of human GIT1 (NP_054749.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GIT1 antibody (144-61053) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] GIT1 Polyclonal Antibody (144-61053)](https://cdn.sanity.io/images/g51potcz/production/6898f0cb09d804940a15ab0af1d9511b8914f5fb-332x350.jpg)
Western blot analysis of extracts from normal (control) and GIT1 knockout (KO) HeLa cells, using GIT1 antibody (144-61053) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] GIT1 Polyclonal Antibody (144-61053)](https://www.raybiotech.com/files/images/Antibodies/144/144-61053/2.jpg)
Expiration:
12 months from the date of shipment when stored properly.