GLUL Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P15104 |
| Gene Id | 2752 |
| Gene Symbols | GLUL |
| Protein Name / Synonyms | GLUL, GLNS, GS, PIG43, PIG59, glutamine synthetase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
42kDa
Observed MW
40kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-373 of human GLUL (NP_001028228.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GLUL antibody (144-05437) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] GLUL Polyclonal Antibody (144-05437)](https://cdn.sanity.io/images/g51potcz/production/2b751878a31a121da0a5e6707de5d3474f2a7fa1-495x350.jpg)
Western blot analysis of extracts from normal (control) and GLUL knockout (KO) HeLa cells, using GLUL antibody (144-05437) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.![Western blot - [KO Validated] GLUL Polyclonal Antibody (144-05437)](https://cdn.sanity.io/images/g51potcz/production/a9a635b41a6919651c6bda1b2667302191f3c0f3-350x350.jpg)
Immunohistochemistry of paraffin-embedded human stomach using GLUL Antibody (144-05437) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] GLUL Polyclonal Antibody (144-05437)](https://cdn.sanity.io/images/g51potcz/production/42836498a728dd00ca4f28dfc1400ffca10051c5-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse lung using GLUL Antibody (144-05437) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] GLUL Polyclonal Antibody (144-05437)](https://cdn.sanity.io/images/g51potcz/production/54c91afde1840d2f1a4d8d7ec4d407c4fae6a289-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse stomach using GLUL Antibody (144-05437) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] GLUL Polyclonal Antibody (144-05437)](https://cdn.sanity.io/images/g51potcz/production/f4bdb87b17aa0e6d5a7b538dfea0614eb2b63e58-366x550.jpg)
Immunofluorescence analysis of MCF-7 cells using GLUL antibody (144-05437). Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] GLUL Polyclonal Antibody (144-05437)](https://www.raybiotech.com/files/images/Antibodies/144/144-05437/6.jpg)
Expiration:
12 months from the date of shipment when stored properly.