[KO Validated] GNAI3 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P08754 |
| Gene Id | 2773 |
| Gene Symbols | GNAI3 |
| Protein Name / Synonyms | GNAI3, 87U6, ARCND1, G protein subunit alpha i3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway.
Dilutions
WB 1:500 - 1:2000
IP 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
40kDa
Observed MW
45kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human GNAI3 (NP_006487.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GNAI3 antibody (144-013307) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] GNAI3 Polyclonal Antibody (144-013307)](https://cdn.sanity.io/images/g51potcz/production/2823856bf5bfb5305374613b05963fcabbd514a7-421x350.jpg)
Western blot analysis of extracts from normal (control) and GNAI3 knockout (KO) HeLa cells, using GNAI3 antibody (144-013307) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] GNAI3 Polyclonal Antibody (144-013307)](https://www.raybiotech.com/files/images/Antibodies/144/144-60276/2.jpg)
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug GNAI3 antibody (144-013307). Western blot was performed from the immunoprecipitate using GNAI3 antibody (144-013307) at a dilition of 1:1000.![Immunoprecipitation - [KO Validated] GNAI3 Polyclonal Antibody (144-013307)](https://www.raybiotech.com/files/images/Antibodies/144/144-60276/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.