GOLM1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q8NBJ4 |
| Gene Id | 51280 |
| Gene Symbols | GOLM1 |
| Protein Name / Synonyms | GOLM1, C9orf155, GOLPH2, GP73, HEL46, PSEC0257, bA379P1.3, Golgi membrane protein 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:50
IHC 1:50 - 1:200
IF 1:20 - 1:50
Format
Calculated MW
44kDa/45kDa
Observed MW
80kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 112-401 of human GOLM1 (NP_057632.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GOLM1 antibody (144-07283) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using GOLM1 antibody (144-07283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using GOLM1 antibody (144-07283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using GOLM1 antibody (144-07283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using GOLM1 antibody (144-07283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using GOLM1 antibody (144-07283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using GOLM1 antibody (144-07283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using GOLM1 antibody (144-07283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using GOLM1 antibody (144-07283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using GOLM1 antibody (144-07283) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.